Mouse FGFR-4/CD334 protein (Recombinant) (C-His) (STJP001464)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Mouse FGFR-4/CD334 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu17-Asp366) of mouse FGFR4/FGF Receptor 4 (Accession #NP_032037.2) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Mouse FGFR-4/CD334-C-His protein was developed in hek293 cells using the region Leu17-Asp366. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its ability to inhibit FGF acidic-dependent proliferation of Balba3T3 mouse fibroblast cells.The ED50 for this effect is 38.74-154.96 ng/mL in the present of mouse FGF-1/aFGF, corresponding to a specific activity of 6.45×10 |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | Fgfr4 |
| Gene ID | 14186 |
| Uniprot ID | FGFR4_MOUSE |
| Immunogen | Recombinant Mouse FGFR-4/CD334 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu17-Asp366) of mouse FGFR4/FGF Receptor 4 (Accession #NP_032037.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Leu17-Asp366 |
| Immunogen Sequence | LSLEASEEMEQEPCLAPILE QQEQVLTVALGQPVRLCCGR TERGRHWYKEGSRLASAGRV RGWRGRLEIASFLPEDAGRY LCLARGSMTVVHNLTLLMDD SLTSISNDEDPKTLSSSSSG HVYPQQAPYWTHPQRMEKKL HAVPAGNTVKFRCPAAGNPM PTIHWLKDGQAFHGENRIGG IRLRHQHWSLVMESVVPSDR GTYTCLVENSLGSIRYSYLL DVLERSPHRPILQAGLPAN |
Additional Info
| Post Translational Modifications | N-glycosylated. Isoform 1 and isoform 2 are glycosylated. Full maturation of the glycan chains in the Golgi is essential for high affinity interaction with FGF19. Ubiquitinated. Subject to proteasomal degradation when not fully glycosylated. Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer. Isoform 1 and isoform 2 are phosphorylated on tyrosine residues. |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation.MMP14 promotes internalization and degradation of FGFR4. Plays a role in postnatal lung development. May be involved in the development of skeletal muscle cell lineages. |
| Protein Name | Fibroblast Growth Factor Receptor 4Fgfr-4Protein-Tyrosine Kinase Receptor Mpk-11Cd Antigen Cd334 |
| Database Links | Reactome: R-MMU-109704Reactome: -MMU-1257604Reactome: -MMU-1307965Reactome: -MMU-190322Reactome: -MMU-5654228Reactome: -MMU-5654712Reactome: -MMU-5654719Reactome: -MMU-5654720Reactome: -MMU-5654733Reactome: -MMU-5673001Reactome: -MMU-6811558 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEndosomeEndoplasmic ReticulumInternalized From The Cell Membrane To Recycling EndosomesAnd From There Back To The Cell Membrane |
| Alternative Protein Names | Fibroblast Growth Factor Receptor 4 proteinFgfr-4 proteinProtein-Tyrosine Kinase Receptor Mpk-11 proteinCd Antigen Cd334 proteinFgfr4 proteinFgfr-4 proteinMpk-11 protein |
Information sourced from Uniprot.org