Mouse Enolase 1/ENO1 protein (Recombinant) (His-Tag) (STJP019356)
SPECIFICATIONS
HostE.coli
ImmunogenMouse
General Information
| Short Description | Recombinant-Mouse Enolase 1/ENO1-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Enzyme Activity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.5) containing 40% Glycerol, 0.1M NaCl |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Specific activity is > 6, 000pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of 2-phosphoglycerate to phosphoenolpyruvate per minute at pH 6.5 at 37C in a couple system with PK and LDH. |
Target Information
| Gene Symbol | Eno1 |
| Gene ID | 13806/433182 |
| Uniprot ID | ENOA_MOUSE |
| Accession Number | NP_075608 |
| Immunogen | Mouse |
| Immunogen Region | 1-434aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Testis. Found in the principal piece of sperm tail (at protein level). The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. In striated muscle, expression of ENO1 appears to be independent of fiber type. |
| Post Translational Modifications | ISGylated. Lysine 2-hydroxyisobutyrylation (Khib) by p300/EP300 activates the phosphopyruvate hydratase activity. |
| Function | Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. |
| Protein Name | Alpha-Enolase2-Phospho-D-Glycerate Hydro-LyaseEnolase 1Non-Neural EnolaseNne |
| Database Links | |
| Cellular Localisation | CytoplasmCell MembraneCan Translocate To The Plasma Membrane In Either The Homodimeric (Alpha/Alpha) Or Heterodimeric (Alpha/Gamma) FormEno1 Is Localized To The M-Band |
| Alternative Protein Names | Alpha-Enolase protein2-Phospho-D-Glycerate Hydro-Lyase proteinEnolase 1 proteinNon-Neural Enolase proteinNne proteinEno1 proteinEno-1 protein |
Information sourced from Uniprot.org