Mouse Dkk-1 protein (Recombinant) (C-His) (STJP001139)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Mouse Dkk-1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Met1-His272) of mouse Dkk-1 (Accession #NP_034181.2. ) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Mouse Dkk-1-C-His protein was developed in hek293 cells using the region Met1-His272. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA.Immobilized Mouse DKK-1 at 5 Mu g/mL (100 Mu L/well) can bind Human LRP-5 with a linear range of 1-399 ng/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | Dkk1 |
| Gene ID | 13380 |
| Uniprot ID | DKK1_MOUSE |
| Immunogen | Recombinant Mouse Dkk-1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Met1-His272) of mouse Dkk-1 (Accession #NP_034181.2. ) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Met1-His272 |
| Immunogen Sequence | MMVVCAAAAVRFLAVFTMMA LCSLPLLGASATLNSVLINS NAIKNLPPPLGGAGGQPGSA VSVAPGVLYEGGNKYQTLDN YQPYPCAEDEECGSDEYCSS PSRGAAGVGGVQICLACRKR RKRCMRHAMCCPGNYCKNGI CMPSDHSHFPRGEIEESIIE NLGNDHNAAAGDGYPRRTTL TSKIYHTKGQEGSVCLRSSD CAAGLCCARHFWSKICKPVL KEGQVCTKHKRKGSHGLEI |
Additional Info
| Function | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity. Plays a role in limb development.attenuates Wnt signaling in the developing limb to allow normal limb patterning. |
| Protein Name | Dickkopf-Related Protein 1Dickkopf-1Dkk-1Mdkk-1 |
| Database Links | Reactome: R-MMU-3772470 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Dickkopf-Related Protein 1 proteinDickkopf-1 proteinDkk-1 proteinMdkk-1 proteinDkk1 protein |
Information sourced from Uniprot.org