| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.214ng/mL |
| Detection Limit: | 0.625-40ng/mL |
| Short Description: | This CYP27B1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse tissue homogenates, cell lysates or other biological fluids.. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | Cyp27b1 |
| Gene ID: | 13115 |
| Uniprot ID: | CP27B_MOUSE |
| Sample Type: | tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Kidney. |
| Post Translational Modifications | |
| Function | A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1-alpha position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR). Has 1-alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway. Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin. |
| Protein Name | 25-Hydroxyvitamin D-1 Alpha Hydroxylase - Mitochondrial 25-Ohd-1 Alpha-Hydroxylase 25-Hydroxyvitamin D(3 1-Alpha-Hydroxylase Vd3 1a Hydroxylase Calcidiol 1-Monooxygenase Cytochrome P450 Subfamily Xxviib Polypeptide 1 Cytochrome P450c1 Alpha Cytochrome P450vd1-Alpha Cytochrome P450 27b1 |
| Database Links | Reactome: R-MMU-196791 Reactome: -MMU-211916 |
| Cellular Localisation | Mitochondrion Membrane |
| Alternative ELISA Names | 25-Hydroxyvitamin D-1 Alpha Hydroxylase - Mitochondrial ELISA kit 25-Ohd-1 Alpha-Hydroxylase ELISA kit 25-Hydroxyvitamin D(3 1-Alpha-Hydroxylase ELISA kit Vd3 1a Hydroxylase ELISA kit Calcidiol 1-Monooxygenase ELISA kit Cytochrome P450 Subfamily Xxviib Polypeptide 1 ELISA kit Cytochrome P450c1 Alpha ELISA kit Cytochrome P450vd1-Alpha ELISA kit Cytochrome P450 27b1 ELISA kit Cyp27b1 ELISA kit Cyp27b ELISA kit Cyp40 ELISA kit |
| output |
Information sourced from Uniprot.org
