Host: | HEK293 |
Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Recombinant-Mouse CTLA4 (CD152)-protein was developed from hek293. For use in research applications. |
Conjugation: | Unconjugated |
Formulation: | Lyophilised from 0.2 Mu m filtered PBS pH 7.4. |
Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
Storage Instruction: | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
Immunoreactivity: | The activity was tested by immobilized recombinant CTLA4 (10ug/ml) ability to bind recombinant Mouse CD80 in functional ELISA. The ED50 was calculated to be 10ng/ml. NA |
Gene Symbol: | Ctla4 |
Gene ID: | 12477 |
Uniprot ID: | CTLA4_MOUSE |
Immunogen Region: | ECD |
Immunogen: | DNA sequence encoding extracellular domain of Mouse CTLA4 to a C-terminal polyHis tag was expressed in HEK cells. NA |
Tissue Specificity | Widely expressed with highest levels in lymphoid tissues. |
Post Translational Modifications | N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. |
Function | Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. |
Protein Name | Cytotoxic T-Lymphocyte Protein 4Cytotoxic T-Lymphocyte-Associated Antigen 4Ctla-4Cd Antigen Cd152 |
Database Links | Reactome: R-MMU-389513 |
Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinExists Primarily An Intracellular Antigen Whose Surface Expression Is Tightly Regulated By Restricted Trafficking To The Cell Surface And Rapid Internalization |
Alternative Protein Names | Cytotoxic T-Lymphocyte Protein 4 proteinCytotoxic T-Lymphocyte-Associated Antigen 4 proteinCtla-4 proteinCd Antigen Cd152 proteinCtla4 proteinCd152 protein |
Information sourced from Uniprot.org