| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: | Measured by its binding ability in a functional ELISA. Immobilized Mouse CTLA-4/CD152 at 2 Mu g/mL (100 Mu L/well) can bind Mouse B7-2/CD86 with a linear range of 0.1-51.8 ng/mL. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Gene Symbol: | Ctla4 |
| Gene ID: | 12477 |
| Uniprot ID: | CTLA4_MOUSE |
| Immunogen: | Recombinant Mouse CTLA-4/CD152 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu36-Asp161) of Mouse CTLA4 (Accession #NP_033973.2) fused with an 6×His tag at the C-terminus. |
| Immunogen Region: | Glu36-Asp161 |
| Immunogen Sequence: | EAIQVTQPSVVLASSHGVAS FPCEYSPSHNTDEVRVTVLR QTNDQMTEVCATTFTEKNTV GFLDYPFCSGTFNESRVNL TIQGLRAVDTGLYLCKVELM YPPPYFVGMGNGTQIYVIDP E PCPDSD |
| Post Translational Modifications | N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. |
| Function | Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. |
| Protein Name | Cytotoxic T-Lymphocyte Protein 4Cytotoxic T-Lymphocyte-Associated Antigen 4Ctla-4Cd Antigen Cd152 |
| Database Links | Reactome: R-MMU-389356Reactome: -MMU-389513 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinExists Primarily An Intracellular Antigen Whose Surface Expression Is Tightly Regulated By Restricted Trafficking To The Cell Surface And Rapid Internalization |
| Alternative Protein Names | Cytotoxic T-Lymphocyte Protein 4 proteinCytotoxic T-Lymphocyte-Associated Antigen 4 proteinCtla-4 proteinCd Antigen Cd152 proteinCtla4 proteinCd152 protein |
Information sourced from Uniprot.org
