| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Mouse CD226 (DNAM-1)-protein was developed from hek293. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: |
Recombinant Mouse CD226 activity was measured using Immobilized mouse CD226 at 2 Mu g/ml ability to bind biotinylated mouse CD155 with a linear ranger of 0.06-0.16 Mu g/ml NA |
| Gene Symbol: |
Cd226 |
| Gene ID: |
225825 |
| Uniprot ID: |
CD226_MOUSE |
| Immunogen Region: |
ECD |
| Immunogen: |
Optimized DNA sequence encoding extracellular domain of Mouse CD226 (DNAM-1) including a C-terminal His tag was expressed in HEK293 cells. NA |
| Post Translational Modifications | Phosphorylated. Phosphorylation of Tyr-319 allows GRB2 binding and activation of subsequent signaling. |
| Function | Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells. Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells. Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes. |
| Protein Name | Cd226 AntigenPlatelet And T-Cell Activation Antigen 1Cd Antigen Cd226 |
| Database Links | Reactome: R-MMU-198933 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinLocalizes To Lipid Rafts |
| Alternative Protein Names | Cd226 Antigen proteinPlatelet And T-Cell Activation Antigen 1 proteinCd Antigen Cd226 proteinCd226 proteinPta1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
