| Function | Precursor of non-enzymatic components of the classical, lectin and GZMK complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Complement C4b-B: Non-enzymatic component of C3 and C5 convertases. Generated following cleavage by complement proteases (C1S, MASP2 or GZMK, depending on the complement pathway), it covalently attaches to the surface of pathogens, where it acts as an opsonin that marks the surface of antigens for removal. It then recruits the serine protease complement C2b to form the C3 and C5 convertases, which cleave and activate C3 and C5, respectively, the next components of the complement pathways. Complement C4b-A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while complement C4b-B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens. C4a anaphylatoxin: Putative humoral mediator released following cleavage by complement proteases (C1S, MASP2 or GZMK, depending on the complement pathway). While it is strongly similar to anaphylatoxins, its role is unclear. Was reported to act as a mediator of local inflammatory process.however these effects were probably due to contamination with C3a and/C5a anaphylatoxins in biological assays. |
| Protein Name | Complement C4-B Cleaved Into - Complement C4 Beta Chain - Complement C4 Alpha Chain - C4a Anaphylatoxin - Complement C4b-B - Complement C4 Gamma Chain |
| Database Links | Reactome: R-MMU-166663Reactome: -MMU-174577Reactome: -MMU-381426Reactome: -MMU-8957275Reactome: -MMU-977606 |
| Cellular Localisation | SecretedC4a Anaphylatoxin: SecretedComplement C4b-B: SecretedCell SurfaceCovalently Associated With The Surface Of Pathogens: The Internal Thioester Bond Reacts With Carbohydrate Antigens On The Target Surface To Form Amide Or Ester Bonds |
| Alternative ELISA Names | Complement C4-B Cleaved Into - Complement C4 Beta Chain - Complement C4 Alpha Chain - C4a Anaphylatoxin - Complement C4b-B - Complement C4 Gamma Chain ELISA kitC4b ELISA kitC4 ELISA kit |
| Specificity | This kit recognizes Mouse C4 in samples. No significant cross-reactivity or interference between Mouse C4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 84-95 | 90 |
| EDTA plasma (n=8) | 97-107 | 101 |
| Cell culture media (n=8) | 100-108 | 104 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 40.17 | 120.98 | 473.35 | 43.16 | 117.19 | 464.94 |
| Standard deviation | 2.03 | 5.86 | 23.43 | 2.49 | 5.46 | 25.48 |
| CV (%) | 5.05 | 4.84 | 4.95 | 5.77 | 4.66 | 5.48 |
![Mouse C4b (Complement C4-B [Cleaved into: Complement C4 beta chain; Complement C4 alpha chain; C4a anaphylatoxin; Complement C4 gamma chain]) Sandwich ELISA Kit (STJE0005336)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/141127/308645/STJE0005336_1__67197.1648490980.png?c=1 "Mouse C4b (Complement C4-B [Cleaved into: Complement C4 beta chain; Complement C4 alpha chain; C4a anaphylatoxin; Complement C4 gamma chain]) Sandwich ELISA Kit (STJE0005336)")
![Mouse C4b (Complement C4-B [Cleaved into: Complement C4 beta chain; Complement C4 alpha chain; C4a anaphylatoxin; Complement C4 gamma chain]) Sandwich ELISA Kit (STJE0005336)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/141127/308645/STJE0005336_1__67197.1648490980.png?c=1 "Mouse C4b (Complement C4-B [Cleaved into: Complement C4 beta chain; Complement C4 alpha chain; C4a anaphylatoxin; Complement C4 gamma chain]) Sandwich ELISA Kit (STJE0005336)")
