Mouse Asparaginyl endopeptidase/Legumain protein (Recombinant) (C-His) (STJP002798)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Mouse Asparaginyl endopeptidase/Legumain Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val18-Tyr435) of mouse Asparaginyl endopeptidase/Legumain (Accession #NP_035305.1) fused with a
General Information
| Short Description | Recombinant-Mouse Asparaginyl endopeptidase/Legumain-C-His protein was developed in hek293 cells using the region Val18-Tyr435. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methyl coumarin (Z-AAN-AMC). The specific activity is > 107 pmol/min/Mu g. |
| Determination Method | <0.1 EU/Mu g |
Target Information
| Gene Symbol | Lgmn |
| Gene ID | 19141 |
| Uniprot ID | LGMN_MOUSE |
| Immunogen | Recombinant Mouse Asparaginyl endopeptidase/Legumain Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val18-Tyr435) of mouse Asparaginyl endopeptidase/Legumain (Accession #NP_035305.1) fused with a |
| Immunogen Region | Val18-Tyr435 |
| Immunogen Sequence | VPVGVDDPEDGGKHWVVIVA GSNGWYNYRHQADACHAYQI IHRNGIPDEQIIVMMYDDIA NSEENPTPGVVINRPNGTDV YKGVLKDYTGEDVTPENFLA VLRGDAEAVKGKGSGKVLKS GPRDHVFIYFTDHGATGILV FPNDDLHVKDLNKTIRYMYE HKMYQKMVFYIEACESGSMM NHLPDDINVYATTAANPKES SYACYYDEERGTYLGDWYSV NWMEDSDVEDLTKETLHKQ |
Additional Info
| Post Translational Modifications | Glycosylated. Activated by autocatalytic processing at pH 4. |
| Function | Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation. |
| Protein Name | LegumainProtease - Cysteine 1 |
| Database Links | Reactome: R-MMU-1679131Reactome: -MMU-2132295 |
| Cellular Localisation | Lysosome |
| Alternative Protein Names | Legumain proteinProtease - Cysteine 1 proteinLgmn proteinPrsc1 protein |
Information sourced from Uniprot.org