| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | <0.1 EU/Mu g |
| Immunoreactivity: | Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methyl coumarin (Z-AAN-AMC). The specific activity is > 107 pmol/min/Mu g. |
| Gene Symbol: | Lgmn |
| Gene ID: | 19141 |
| Uniprot ID: | LGMN_MOUSE |
| Immunogen Region: | Val18-Tyr435 |
| Immunogen: | Recombinant Mouse Asparaginyl endopeptidase/Legumain Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val18-Tyr435) of mouse Asparaginyl endopeptidase/Legumain (Accession #NP_035305.1) fused with a |
| Immunogen Sequence: | VPVGVDDPEDGGKHWVVIVA GSNGWYNYRHQADACHAYQI IHRNGIPDEQIIVMMYDDIA NSEENPTPGVVINRPNGTDV YKGVLKDYTGEDVTPENFLA VLRGDAEAVKGKGSGKVLKS GPRDHVFIYFTDHGATGILV FPNDDLHVKDLNKTIRYMYE HKMYQKMVFYIEACESGSMM NHLPDDINVYATTAANPKES SYACYYDEERGTYLGDWYSV NWMEDSDVEDLTKETLHKQ |
| Post Translational Modifications | Glycosylated. Activated by autocatalytic processing at pH 4. |
| Function | Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation. |
| Protein Name | LegumainProtease - Cysteine 1 |
| Database Links | Reactome: R-MMU-1679131Reactome: -MMU-2132295 |
| Cellular Localisation | Lysosome |
| Alternative Protein Names | Legumain proteinProtease - Cysteine 1 proteinLgmn proteinPrsc1 protein |
Information sourced from Uniprot.org
