| Host: |
HEK293 cells |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
< 1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: |
Measured by its binding ability in a functional ELISA. Immobilized Mouse APOE at 5 Mu g/mL (100 Mu L/well) can bind Mouse TREM2 with a linear range of 0.16-1.5 Mu g/mL. |
| Gene Symbol: |
Apoe |
| Gene ID: |
11816 |
| Uniprot ID: |
APOE_MOUSE |
| Immunogen Region: |
Glu19-Gln311 |
| Immunogen: |
Recombinant Mouse ApoE/APOE/Apolipoprotein E Protein is produced by Human cells expression system. The target protein is expressed with sequence (Glu19-Gln311) of mouse ApoE/APOE/Apolipoprotein E (Accession #P08226) fused with a 6×His tag at the C-te |
| Immunogen Sequence: |
EGEPEVTDQLEWQSNQPWEQ ALNRFWDYLRWVQTLSDQVQ EELQSSQVTQELTALMEDTM TEVKAYKKELEEQLGPVAEE TRARLGKEVQAAQARLGADM EDLRNRLGQYRNEVHTMLGQ STEEIRARLSTHLRKMRKRL MRDAEDLQKRLAVYKAGARE GAERGVSAIRERLGPLVEQG RQRTANLGAGAAQPLRDRAQ AFGDRIRGRLEEVGNQARDR LEEVREHMEEVRSKMEEQT |
| Function | APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells. |
| Protein Name | Apolipoprotein EApo-E |
| Database Links | Reactome: R-MMU-3000480Reactome: -MMU-381426Reactome: -MMU-8957275Reactome: -MMU-8963888Reactome: -MMU-8963901Reactome: -MMU-8964026Reactome: -MMU-8964058Reactome: -MMU-975634 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixExtracellular VesicleEndosomeMultivesicular BodyIn The PlasmaApoe Is Associated With ChylomicronsChylomicrons RemnantsVldlLdl And Hdl LipoproteinsLipid Poor Oligomeric Apoe Is Associated With The Extracellular Matrix In A Calcium- And Heparan-Sulfate Proteoglycans-Dependent MannerLipidation Induces The Release From The Extracellular MatrixColocalizes With Cd63 And Pmel At Exosomes And In Intraluminal Vesicles Within Multivesicular Endosomes |
| Alternative Protein Names | Apolipoprotein E proteinApo-E proteinApoe protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
