Mouse ALCAM (Cd166 Antigen) Antibody Pair (STJAP00555)
General Information
| Short Description | This ALCAM antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | Alcam |
| Gene ID | 11658 |
| Uniprot ID | CD166_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse ALCAM protien.Detection Immunogen: Recombinant Mouse ALCAM protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Glycosylated. |
| Function | Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6. Contributes to the formation and maturation of the immunological synapse via its interactions with CD6. Mediates homotypic interactions with cells that express ALCAM. Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis.required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation. Promotes neurite extension, axon growth and axon guidance.axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons. |
| Protein Name | Cd166 AntigenActivated Leukocyte Cell Adhesion MoleculeBenProtein Dm-GraspCd Antigen Cd166 |
| Database Links | |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionAxonDendriteDetected At The Immunological SynapseIEAt The Contact Zone Between Antigen-Presenting Dendritic Cells And T-CellsColocalizes With Cd6 And The Tcr/Cd3 Complex At The Immunological Synapse |
| Alternative Antibody Pair Names | Cd166 Antigen antibody pairActivated Leukocyte Cell Adhesion Molecule antibody pairBen antibody pairProtein Dm-Grasp antibody pairCd Antigen Cd166 antibody pairAlcam antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse ALCAM (Cd166 Antigen) Antibody Pair (STJAP00555)
This antibody pair is designed to detect mouse ALCAM (CD166 Antigen), corresponding to UniProt entry Q61490. Reactivity has been confirmed for mouse samples only.
The capture antibody is supplied unconjugated, while the detection antibody is biotin-conjugated. Both are raised in goat, purified by antigen affinity, and supplied at 0.5 mg/mL.
This pair is validated for use in ELISA. It has not been described as validated for other assay formats such as lateral flow or other immunoassay platforms.
The pair is suitable for use with serum, plasma, and other biological fluids of mouse origin.
Both the capture and detection antibodies were raised against recombinant mouse ALCAM protein. Both antibodies were subsequently purified by antigen affinity chromatography.
Both components should be stored at -20°C for long-term storage, and freeze-thaw cycles should be avoided. Each antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, with the detection antibody also containing 1% protective protein.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.