| Post Translational Modifications | Glycosylated.contains two glycans. Sulfated. |
| Function | Binds estrogens, fatty acids and metals. |
| Protein Name | Alpha-FetoproteinAlpha-1-FetoproteinAlpha-Fetoglobulin |
| Database Links | Reactome: R-MMU-381426Reactome: -MMU-8957275 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Alpha-Fetoprotein ELISA kitAlpha-1-Fetoprotein ELISA kitAlpha-Fetoglobulin ELISA kitAfp ELISA kit |
| Specificity | This kit recognizes Mouse αFP in samples. No significant cross-reactivity or interference between Mouse αFP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-101 | 96 |
| EDTA plasma (n=8) | 95-108 | 101 |
| Cell culture media (n=8) | 92-105 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.50 | 1.30 | 4.60 | 0.50 | 1.20 | 5.00 |
| Standard deviation | 0.03 | 0.07 | 0.16 | 0.03 | 0.07 | 0.23 |
| CV (%) | 6.00 | 5.38 | 3.48 | 6.00 | 5.83 | 4.60 |
