| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 46.88pg/mL |
| Detection Limit: | 78.13~5000pg/mL |
| Short Description : | This ACV-A Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Inhba |
| Gene ID: | 16323 |
| Uniprot ID: | INHBA_MOUSE |
| Specificity: | This kit recognizes Mouse ACV-A in samples. No significant cross-reactivity or interference between Mouse ACV-A and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. |
| Protein Name | Inhibin Beta A Chain Activin Beta-A Chain |
| Database Links | Reactome: R-MMU-1502540 Reactome: -MMU-201451 Reactome: -MMU-209822 Reactome: -MMU-2473224 Reactome: -MMU-9839406 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Inhibin Beta A Chain ELISA kit Activin Beta-A Chain ELISA kit Inhba ELISA kit |
| Specificity | This kit recognizes Mouse ACV-A in samples. No significant cross-reactivity or interference between Mouse ACV-A and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-110 | 100 |
| EDTA plasma (n=8) | 95-108 | 101 |
| Cell culture media (n=8) | 94-109 | 100 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 269.70 | 767.83 | 2199.56 | 275.09 | 840.71 | 2025.95 |
| Standard deviation | 16.88 | 44.46 | 69.95 | 14.28 | 42.88 | 81.04 |
| CV (%) | 6.26 | 5.79 | 3.18 | 5.19 | 5.10 | 4.00 |