| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Phospho-JAK2 Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Dilution Range: |
WB: 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Tissue Specificity | Ubiquitously expressed throughout most tissues. |
| Post Translational Modifications | Autophosphorylated, leading to regulate its activity. Leptin promotes phosphorylation on tyrosine residues, including phosphorylation on Tyr-813. Autophosphorylation on Tyr-119 in response to EPO down-regulates its kinase activity. Autophosphorylation on Tyr-868, Tyr-966 and Tyr-972 in response to growth hormone (GH) are required for maximal kinase activity. Also phosphorylated by TEC. Phosphorylated on tyrosine residues in response to interferon gamma signaling. Phosphorylated on tyrosine residues in response to a signaling cascade that is activated by increased cellular retinol. Undergoes Notch-induced ubiquitination and subsequent proteasomal degradation which is mediated by ASB1 or ASB2, the substrate-recognition components of probable ECS E3 ubiquitin-protein ligase complexes. |
| Function | Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO).or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin. |
| Peptide Name | Tyrosine-Protein Kinase Jak2Janus Kinase 2Jak-2 |
| Database Links | Reactome: R-HSA-1059683Reactome: R-HSA-110056Reactome: R-HSA-112411Reactome: R-HSA-1170546Reactome: R-HSA-1433557Reactome: R-HSA-2586552Reactome: R-HSA-3214858Reactome: R-HSA-512988Reactome: R-HSA-5673000Reactome: R-HSA-5673001Reactome: R-HSA-6785807Reactome: R-HSA-6788467Reactome: R-HSA-6802946Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-69231Reactome: R-HSA-877300Reactome: R-HSA-877312Reactome: R-HSA-8854691Reactome: R-HSA-8984722Reactome: R-HSA-9006335Reactome: R-HSA-9020591Reactome: R-HSA-9020933Reactome: R-HSA-9020956Reactome: R-HSA-9027276Reactome: R-HSA-9027277Reactome: R-HSA-9027283Reactome: R-HSA-9027284Reactome: R-HSA-912526Reactome: R-HSA-9649948Reactome: R-HSA-9656223Reactome: R-HSA-9670439Reactome: R-HSA-9674555Reactome: R-HSA-9679191Reactome: R-HSA-9705462Reactome: R-HSA-9732724Reactome: R-HSA-982772Reactome: R-HSA-983231 |
| Cellular Localisation | Endomembrane SystemPeripheral Membrane ProteinCytoplasmNucleus |
| Alternative Peptide Names | Tyrosine-Protein Kinase Jak2 proteinJanus Kinase 2 proteinJak-2 proteinJAK2 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
