| Applications: |
Immunodepletion/Immunocompetition |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
IRE1 Blocking Peptide is synthetically produced from the 900-1000 sequence and is suitable for use in western blot applications. |
| Formulation: |
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
ERN1 |
| Gene ID: |
2081 |
| Uniprot ID: |
ERN1_HUMAN |
| Immunogen Region: |
900-1000 |
| Immunogen: |
Synthetic peptide corresponding to unique amino acid sequence within amino acid region 900-1000 on human IRE1A protein. |
| Tissue Specificity | Ubiquitously expressed. High levels observed in pancreatic tissue. |
| Post Translational Modifications | Autophosphorylated following homodimerization. Autophosphorylation promotes activation of the endoribonuclease domain. In response to ER stress, phosphorylated at Ser-724, Ser-729 and possibly Ser-726.phosphorylation promotes oligomerization and endoribonuclease activity. Dephosphorylated at Ser-724, Ser-729 and possibly Ser-726 by RPAP2 to abort failed ER-stress adaptation and trigger apoptosis. ADP-ribosylated by PARP16 upon ER stress, which increases both kinase and endonuclease activities. |
| Function | Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity. The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA. The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes. Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A. |
| Peptide Name | Serine/Threonine-Protein Kinase/Endoribonuclease Ire1Endoplasmic Reticulum-To-Nucleus Signaling 1Inositol-Requiring Protein 1Hire1pIre1-AlphaIre1a Includes - Serine/Threonine-Protein Kinase - Endoribonuclease |
| Database Links | Reactome: R-HSA-381070 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type I Membrane Protein |
| Alternative Peptide Names | Serine/Threonine-Protein Kinase/Endoribonuclease Ire1 proteinEndoplasmic Reticulum-To-Nucleus Signaling 1 proteinInositol-Requiring Protein 1 proteinHire1p proteinIre1-Alpha proteinIre1a Includes - Serine/Threonine-Protein Kinase - Endoribonuclease proteinERN1 proteinIRE1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
