| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.27ng/mL |
| Detection Limit: | 0.781-50ng/mL |
| Short Description: | This WWP2 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids.. |
| Storage Instruction: | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 3 hrs |
| Gene Symbol: | WWP2 |
| Gene ID: | 11060 |
| Uniprot ID: | WWP2_HUMAN |
| Immunogen Region: | Ready-To-Use |
| Sample Type: | tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Detected in heart, throughout the brain, placenta, lung, liver, muscle, kidney and pancreas. Also detected in spleen and peripheral blood leukocytes. |
| Post Translational Modifications | Autoubiquitinated. Ubiquitinated by the SCF(FBXL15) complex, leading to its degradation by the proteasome. |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation.in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation.in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2.the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation. |
| Protein Name | Nedd4-Like E3 Ubiquitin-Protein Ligase Wwp2 Atrophin-1-Interacting Protein 2 Aip2 Hect-Type E3 Ubiquitin Transferase Wwp2 Ww Domain-Containing Protein 2 |
| Database Links | Reactome: R-HSA-8948751 Reactome: R-HSA-9013406 Reactome: R-HSA-9013409 Reactome: R-HSA-9013420 Reactome: R-HSA-9013507 |
| Cellular Localisation | Nucleus |
| Alternative ELISA Names | Nedd4-Like E3 Ubiquitin-Protein Ligase Wwp2 ELISA kit Atrophin-1-Interacting Protein 2 ELISA kit Aip2 ELISA kit Hect-Type E3 Ubiquitin Transferase Wwp2 ELISA kit Ww Domain-Containing Protein 2 ELISA kit WWP2 ELISA kit |
| output |
Information sourced from Uniprot.org
