| Host: | E. coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human WNT5A-N-His protein was developed from e. coli and has a target region of N-His. For use in research applications. |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Gene Symbol: | WNT5A |
| Gene ID: | 7474 |
| Uniprot ID: | WNT5A_HUMAN |
| Immunogen Region: | Ile62-Lys380 |
| Immunogen: | Homo sapiens (Human) |
| Post Translational Modifications | Glycosylation is necessary for secretion but not for activity. Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition. Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT5A. |
| Function | Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes. |
| Protein Name | Protein Wnt-5a |
| Database Links | Reactome: R-HSA-201681Reactome: R-HSA-3238698Reactome: R-HSA-373080Reactome: R-HSA-3772470Reactome: R-HSA-4086398Reactome: R-HSA-4086400Reactome: R-HSA-4608870Reactome: R-HSA-5099900Reactome: R-HSA-5140745Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-9673324 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Protein Names | Protein Wnt-5a proteinWNT5A protein |
Information sourced from Uniprot.org
