Human WNT3A (Active) protein (Recombinant-Active) (STJP012615)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Active-Human WNT3A (Active)-protein was developed from mammalian cells. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | 25mM Tris-HCl, 500mM NaCl, pH 8.2, 5% Trehalose, 2% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at ≤-80°C, stable for 1 year after receipt. Store at ≤-80°C, stable for 3 months under sterile conditions after opening. |
| Endotoxin | < 0.01 EU/µg as determined by LAL test. |
Target Information
| Gene Symbol | WNT3A |
| Gene ID | 89780 |
| Uniprot ID | WNT3A_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Ser19-Lys352 |
Additional Info
| Post Translational Modifications | Palmitoleoylation by PORCN is required for efficient binding to frizzled receptors. Palmitoleoylation is required for proper trafficking to cell surface, vacuolar acidification is critical to release palmitoleoylated WNT3A from WLS in secretory vesicles. Depalmitoleoylated by NOTUM, leading to inhibit Wnt signaling pathway, possibly by promoting disulfide bond formation and oligomerization. Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT3A. Disulfide bonds have critical and distinct roles in secretion and activity. Loss of each conserved cysteine in WNT3A results in high molecular weight oxidized Wnt oligomers, which are formed through inter-Wnt disulfide bonding. |
| Function | Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube. Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro). |
| Protein Name | Protein Wnt-3a |
| Database Links | Reactome: R-HSA-201681Reactome: R-HSA-3238698Reactome: R-HSA-373080Reactome: R-HSA-3772470Reactome: R-HSA-4641262Reactome: R-HSA-4641263Reactome: R-HSA-5340588Reactome: R-HSA-9793380Reactome: R-HSA-9832991Reactome: R-HSA-9834899Reactome: R-HSA-9856649 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Protein Names | Protein Wnt-3a proteinWNT3A protein |
Information sourced from Uniprot.org