Human VRK3 protein (Recombinant) (His-Tag) (STJP017695)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human VRK3-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.25 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 40% Glycerol, 0.15M NaCl, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | VRK3 |
| Gene ID | 51231 |
| Uniprot ID | VRK3_HUMAN |
| Accession Number | NP_057524 |
| Immunogen | Human |
| Immunogen Region | 1-474aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MGSMISFCPD CGKSIQAAFK FCPYCGNSLP VEEHVGSQTF VNPHVSSFQG SKRGLNSSFE TSPKKVKWSS TVTSPRLSLF SDGDSSESED TLSSSERSKG SGSRPPTPKS SPQKTRKSPQ VTRGSPQKTS CSPQKTRQSP QTLKRSRVTT SLEALPTGTV LTDKSGRQWK LKSFQTRDNQ GILYEAAPTS TLTCDSGPQK QKFSLKLD |
Additional Info
| Post Translational Modifications | Phosphorylated at Ser-108 by CDK5.leading to protection of the cell against H2O2-induced apoptosis. Ubiquitinated by RNF144A. |
| Function | Plays a role in the regulation of the cell cycle by phosphorylating the nuclear envelope protein barrier-to-autointegration factor/BAF that is required for disassembly and reassembly, respectively, of the nuclear envelope during mitosis. Under normal physiological conditions, negatively regulates ERK activity along with VHR/DUSP3 phosphatase in the nucleus, causing timely and transient action of ERK. Stress conditions activate CDK5 which phosphorylates VRK3 to increase VHR phosphatase activity and suppress prolonged ERK activation that causes cell death. For example, upon glutamate induction, promotes nuclear localization of HSP70/HSPA1A to inhibit ERK activation via VHR/DUSP3 phosphatase. |
| Protein Name | Serine/Threonine-Protein Kinase Vrk3Vaccinia-Related Kinase 3 |
| Database Links | Reactome: R-HSA-202670 |
| Cellular Localisation | NucleusCytoplasmUnder Oxidative StressMigrates From The Nucleus To The Cytoplasm |
| Alternative Protein Names | Serine/Threonine-Protein Kinase Vrk3 proteinVaccinia-Related Kinase 3 proteinVRK3 protein |
Information sourced from Uniprot.org