| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.062ng/mL |
| Detection Limit: | 0.156-10ng/mL |
| Short Description: | This vHL Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.. |
| Storage Instruction: | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 3 hrs |
| Gene Symbol: | VHL |
| Gene ID: | 7428 |
| Uniprot ID: | VHL_HUMAN |
| Immunogen Region: | Ready-To-Use |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | Expressed in the adult and fetal brain and kidney. |
| Post Translational Modifications | |
| Function | Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2. Acts as a negative regulator of mTORC1 by promoting ubiquitination and degradation of RPTOR. |
| Protein Name | Von Hippel-Lindau Disease Tumor Suppressor Protein G7 Pvhl |
| Database Links | Reactome: R-HSA-1234176 Reactome: R-HSA-3232142 Reactome: R-HSA-8951664 Reactome: R-HSA-9682706 Reactome: R-HSA-9694686 Reactome: R-HSA-9706019 Reactome: R-HSA-983168 |
| Cellular Localisation | Isoform 1: Cytoplasm Cell Membrane Peripheral Membrane Protein Endoplasmic Reticulum Nucleus Found Predominantly In The Cytoplasm And With Less Amounts Nuclear Or Membrane-Associated Colocalizes With Adrb2 At The Cell Membrane Isoform 3: Cytoplasm Equally Distributed Between The Nucleus And The Cytoplasm But Not Membrane-Associated |
| Alternative ELISA Names | Von Hippel-Lindau Disease Tumor Suppressor ELISA kit Protein G7 ELISA kit Pvhl ELISA kit VHL ELISA kit |
| output |
Information sourced from Uniprot.org
![Anti-Von Hippel Lindau/VHL antibody [SM5057] (STJA0005057)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/180114/403385/STJA0005057_1__84621.1738950448.png?c=1 "Anti-Von Hippel Lindau/VHL antibody [SM5057] (STJA0005057)")