Human VEGFR3/Flt4 (Vascular Endothelial Growth Factor Receptor 3) Antibody Pair (STJAP00410)
General Information
| Short Description | This VEGFR3/Flt4 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A.Detection Purification: Protein A. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | FLT4 |
| Gene ID | 2324 |
| Uniprot ID | VGFR3_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human VEGFR3/Flt4 protien.Detection Immunogen: Recombinant Human VEGFR3/Flt4 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation in response to H(2)O(2) is mediated by a process that requires SRC and PRKCD activity. Phosphorylation at Tyr-1068 is required for autophosphorylation at additional tyrosine residues. Phosphorylation at Tyr-1063 and Tyr-1337 is important for interaction with CRK and subsequent activation of MAPK8. Phosphorylation at Tyr-1230, Tyr-1231 and Tyr-1337 is important for interaction with GRB2 and subsequent activation of the AKT1 and MAPK1/ERK2 and/or MAPK3/ERK1 signaling pathways. In response to endothelial cell adhesion onto collagen, can also be phosphorylated in the absence of FLT4 kinase activity by SRC at Tyr-830, Tyr-833, Tyr-853, Tyr-1063, Tyr-1333, and Tyr-1337. |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades.isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'. |
| Protein Name | Vascular Endothelial Growth Factor Receptor 3Vegfr-3Fms-Like Tyrosine Kinase 4Flt-4Tyrosine-Protein Kinase Receptor Flt4 |
| Database Links | Reactome: R-HSA-195399Reactome: R-HSA-9013695Reactome: R-HSA-9856530 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCytoplasmNucleusLigand-Mediated Autophosphorylation Leads To Rapid InternalizationIsoform 1: Cell MembraneIsoform 2: Cell MembraneIsoform 3: Secreted |
| Alternative Antibody Pair Names | Vascular Endothelial Growth Factor Receptor 3 antibody pairVegfr-3 antibody pairFms-Like Tyrosine Kinase 4 antibody pairFlt-4 antibody pairTyrosine-Protein Kinase Receptor Flt4 antibody pairFLT4 antibody pairVEGFR3 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human VEGFR3/Flt4 (Vascular Endothelial Growth Factor Receptor 3) Antibody Pair (STJAP00410)
This antibody pair detects human VEGFR3/Flt4 (UniProt P35916) and is validated for human samples only. Reactivity in other species has not been confirmed for this product.
The capture antibody is supplied unconjugated, while the detection antibody is biotin-conjugated. Both are mouse-derived, purified by Protein A, and supplied at 0.5 mg/mL.
This pair is validated for use in ELISA. Suitable sample types include serum, plasma, and other biological fluids.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain product integrity.
Both the capture and detection antibodies were raised against recombinant human VEGFR3/Flt4 protein. No specific immunogen region is defined in the product details.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.