Human VCAM-1/CD106 protein (Recombinant) (C-His) (STJP001072)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human VCAM-1/CD106 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Phe25-Glu698) of human VCAM1 (Accession #NP_001069.1) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human VCAM-1/CD106-C-His protein was developed in hek293 cells using the region Phe25-Glu698. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to human VCAM1 coated plates (10 Mu g/mL with 100 Mu L/well) , approximately 80%-90% cells will ad |
| Determination Method | Please contact us for more information. |
Target Information
| Gene Symbol | VCAM1 |
| Gene ID | 7412 |
| Uniprot ID | VCAM1_HUMAN |
| Immunogen | Recombinant Human VCAM-1/CD106 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Phe25-Glu698) of human VCAM1 (Accession #NP_001069.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Phe25-Glu698 |
| Immunogen Sequence | FKIETTPESRYLAQIGDSVS LTCSTTGCESPFFSWRTQID SPLNGKVTNEGTTSTLTMNP VSFGNEHSYLCTATCESRKL EKGIQVEIYSFPKDPEIHLS GPLEAGKPITVKCSVADVYP FDRLEIDLLKGDHLMKSQEF LEDADRKSLETKSLEVTFTP VIEDIGKVLVCRAKLHIDEM DSVPTVRQAVKELQVYISPK NTVISVNPSTKLQEGGSVTM TCSSEGLPAPEIFWSKKLD |
Additional Info
| Post Translational Modifications | Cleaved by the metalloproteinase ADAM17 to generate the soluble form. Sialoglycoprotein. Ubiquitinated by TRIM65 via 'Lys-48'-linked ubiquitination.leading to proteasomal degradation. |
| Function | Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Also serves as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation. |
| Protein Name | Vascular Cell Adhesion Protein 1V-Cam 1Vcam-1Incam-100Cd Antigen Cd106 Cleaved Into - Soluble Vascular Cell Adhesion Molecule-1 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-216083Reactome: R-HSA-6785807Reactome: R-HSA-877300 |
| Cellular Localisation | Vascular Cell Adhesion Protein 1: Cell MembraneSingle-Pass Type I Membrane ProteinSoluble Vascular Cell Adhesion Molecule-1: Secreted |
| Alternative Protein Names | Vascular Cell Adhesion Protein 1 proteinV-Cam 1 proteinVcam-1 proteinIncam-100 proteinCd Antigen Cd106 Cleaved Into - Soluble Vascular Cell Adhesion Molecule-1 proteinVCAM1 protein |
Information sourced from Uniprot.org