| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | Please contact us for more information. |
| Immunoreactivity: | Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to human VCAM1 coated plates (10 Mu g/mL with 100 Mu L/well) , approximately 80%-90% cells will ad |
| Gene Symbol: | VCAM1 |
| Gene ID: | 7412 |
| Uniprot ID: | VCAM1_HUMAN |
| Immunogen: | Recombinant Human VCAM-1/CD106 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Phe25-Glu698) of human VCAM1 (Accession #NP_001069.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | FKIETTPESRYLAQIGDSVS LTCSTTGCESPFFSWRTQID SPLNGKVTNEGTTSTLTMNP VSFGNEHSYLCTATCESRKL EKGIQVEIYSFPKDPEIHLS GPLEAGKPITVKCSVADVYP FDRLEIDLLKGDHLMKSQEF LEDADRKSLETKSLEVTFTP VIEDIGKVLVCRAKLHIDEM DSVPTVRQAVKELQVYISPK NTVISVNPSTKLQEGGSVTM TCSSEGLPAPEIFWSKKLD |
| Post Translational Modifications | Cleaved by the metalloproteinase ADAM17 to generate the soluble form. Sialoglycoprotein. Ubiquitinated by TRIM65 via 'Lys-48'-linked ubiquitination.leading to proteasomal degradation. |
| Function | Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation. |
| Protein Name | Vascular Cell Adhesion Protein 1 V-Cam 1 Vcam-1 Incam-100 Cd Antigen Cd106 Cleaved Into - Soluble Vascular Cell Adhesion Molecule-1 |
| Database Links | Reactome: R-HSA-198933 Reactome: R-HSA-216083 Reactome: R-HSA-6785807 Reactome: R-HSA-877300 |
| Cellular Localisation | Vascular Cell Adhesion Protein 1: Cell Membrane Single-Pass Type I Membrane Protein Soluble Vascular Cell Adhesion Molecule-1: Secreted |
| Alternative Protein Names | Vascular Cell Adhesion Protein 1 protein V-Cam 1 protein Vcam-1 protein Incam-100 protein Cd Antigen Cd106 Cleaved Into - Soluble Vascular Cell Adhesion Molecule-1 protein VCAM1 protein |
Information sourced from Uniprot.org