Human USP30 protein (Recombinant) (N-His) (STJP011022)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human USP30-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | USP30 |
| Gene ID | 84749 |
| Uniprot ID | UBP30_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Gly74-Lys355 |
Additional Info
| Post Translational Modifications | Ubiquitinated by parkin (PRKN) at Lys-235 and Lys-289, leading to its degradation. |
| Function | Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy. Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling. Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65'. Acts as a negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 30Deubiquitinating Enzyme 30Ubiquitin Thioesterase 30Ubiquitin-Specific-Processing Protease 30Ub-Specific Protease 30 |
| Database Links | Reactome: R-HSA-5689880Reactome: R-HSA-9664873 |
| Cellular Localisation | Mitochondrion Outer Membrane |
| Alternative Protein Names | Ubiquitin Carboxyl-Terminal Hydrolase 30 proteinDeubiquitinating Enzyme 30 proteinUbiquitin Thioesterase 30 proteinUbiquitin-Specific-Processing Protease 30 proteinUb-Specific Protease 30 proteinUSP30 protein |
Information sourced from Uniprot.org