SDS-PAGE gel of recombinant human UCHL3 protein under reducing conditions stained with Coomassie blue

Human UCHL3 protein (Recombinant) (His-Tag) (STJP017010)

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STJP017010
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Short Description :Recombinant-Human UCHL3-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
Applications:SDS-PAGE/Enzyme Activity
Host:E.coli
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Concentration:1 mg/mL
Formulation:Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% Glycerol
Storage Instruction:For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.
Immunoreactivity:Specific activity is > 1, 500pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0pmole of ubiquitin-AMC per minute at pH 7.5 at 37C.
Gene Symbol:UCHL3
Gene ID:7347
Uniprot ID:UCHL3_HUMAN
Accession Number:NP_005993
Immunogen:Human
Immunogen Region:1-230aa
Immunogen Sequence:MGSSHHHHHH SSGLVPRGSH MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEV
Tissue Specificity Highly expressed in heart, skeletal muscle, and testis.
Function Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3'', and exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3
Uch-L3
Ubiquitin Thioesterase L3
Database Links Reactome: R-HSA-5689603
Reactome: R-HSA-8866652
Reactome: R-HSA-8951664
Cellular Localisation Cytoplasm
Alternative Protein Names Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3 protein
Uch-L3 protein
Ubiquitin Thioesterase L3 protein
UCHL3 protein

Information sourced from Uniprot.org

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