| Host: |
E.coli |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Lyophilised from a 0.22 Mu m filtered solution of 20mM Tris-HCl, 250mM NaCl, 0.05% Tween80, 1mM DTT, pH 8.5. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
< 1 EU/Mu g of the protein by LAL method. |
| Gene Symbol: |
UCHL1 |
| Gene ID: |
7345 |
| Uniprot ID: |
UCHL1_HUMAN |
| Immunogen Region: |
Met1-Ala223 |
| Immunogen: |
Recombinant Human Ubiquitin thioesterase L1/UCHL1 protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Ala223) of Human Ubiquitin thioesterase L1/UCHL1 (Accession #NP_004172.2) fused with a His |
| Immunogen Sequence: |
MSPILGYWKIKGLVQPTRLL LEYLEEKYEEHLYERDEGDK WRNKKFELGLEFPNLPYYID GDVKLTQSMAIIRYIADKHN MLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKV DFLSKLPEMLKMFEDRLCHK TYLNGDHVTHPDFMLYDALD VVLYMDPMCLDAFPKLVCFK KRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPK |
| Tissue Specificity | Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. |
| Post Translational Modifications | O-glycosylated. |
| Function | Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis. Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1. In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways. Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production. Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes. Mediates the 'Lys-48'-linked deubiquitination of the transcriptional coactivator WWTR1/TAZ leading to its stabilization and inhibition of osteoclastogenesis. Deubiquitinates and stabilizes epidermal growth factor receptor EGFR to prevent its degradation and to activate its downstream mediators. Modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins. Enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by abrogateing its VHL E3 ligase-mediated ubiquitination and consequently inhibiting its degradation. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1Uch-L1Neuron Cytoplasmic Protein 9.5Pgp 9.5Pgp9.5Ubiquitin Thioesterase L1 |
| Database Links | Reactome: R-HSA-5689603 |
| Cellular Localisation | CytoplasmEndoplasmic Reticulum MembraneLipid-AnchorAbout 30% Of Total Uchl1 Is Associated With Membranes In BrainLocalizes Near And/Or Within Mitochondria To Potentially Interact With Mitochondrial Proteins |
| Alternative Protein Names | Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 proteinUch-L1 proteinNeuron Cytoplasmic Protein 9.5 proteinPgp 9.5 proteinPgp9.5 proteinUbiquitin Thioesterase L1 proteinUCHL1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
