• Human UBE3A (Ubiquitin-protein ligase E3A) CLIA Kit (STJC0000572)

Human UBE3A (Ubiquitin-protein ligase E3A) CLIA Kit (STJC0000572)

SKU:
STJC0000572

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This human UBE3A kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: UBE3A
Gene ID: 7337
Uniprot ID: UBE3A_HUMAN
Specificity: This kit recognizes Human UBE3A in samples. No significant cross-reactivity or interference between Human UBE3A and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Phosphorylation at Tyr-659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells.
Function E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the BMAL1, ARC, LAMTOR1, RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component BMAL1, leading to its proteasomal degradation. Acts as transcriptional coactivator of progesterone receptor PGR upon progesterone hormone activation. Acts as a regulator of synaptic development by mediating ubiquitination and degradation of ARC. Required for synaptic remodeling in neurons by mediating ubiquitination and degradation of LAMTOR1, thereby limiting mTORC1 signaling and activity-dependent synaptic remodeling. Synergizes with WBP2 in enhancing PGR activity. (Microbial infection) Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses.
Protein Name Ubiquitin-Protein Ligase E3a
E6ap Ubiquitin-Protein Ligase
Hect-Type Ubiquitin Transferase E3a
Human Papillomavirus E6-Associated Protein
Oncogenic Protein-Associated Protein E6-Ap
Renal Carcinoma Antigen Ny-Ren-54
Database Links Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Nucleus
Alternative CLIA Names Ubiquitin-Protein Ligase E3a CLIA kit
E6ap Ubiquitin-Protein Ligase CLIA kit
Hect-Type Ubiquitin Transferase E3a CLIA kit
Human Papillomavirus E6-Associated Protein CLIA kit
Oncogenic Protein-Associated Protein E6-Ap CLIA kit
Renal Carcinoma Antigen Ny-Ren-54 CLIA kit
UBE3A CLIA kit
E6AP CLIA kit
EPVE6AP CLIA kit
HPVE6A CLIA kit
Specificity This kit recognizes Human UBE3A in samples. No significant cross-reactivity or interference between Human UBE3A and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-106 99
EDTA plasma(n=8) 83-99 90
Cell culture media(n=8) 96-113 103

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 186.53 343.31 1638.06 198.04 310.29 1602.45
Standard deviation 18.26 24.07 167.90 20.30 32.08 177.07
CV (%) 9.79 7.01 10.25 10.25 10.34 11.05
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance