| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human UBE3A kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
UBE3A |
| Gene ID: |
7337 |
| Uniprot ID: |
UBE3A_HUMAN |
| Specificity: |
This kit recognizes Human UBE3A in samples. No significant cross-reactivity or interference between Human UBE3A and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylation at Tyr-659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells. |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the BMAL1, ARC, LAMTOR1, RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component BMAL1, leading to its proteasomal degradation. Acts as transcriptional coactivator of progesterone receptor PGR upon progesterone hormone activation. Acts as a regulator of synaptic development by mediating ubiquitination and degradation of ARC. Required for synaptic remodeling in neurons by mediating ubiquitination and degradation of LAMTOR1, thereby limiting mTORC1 signaling and activity-dependent synaptic remodeling. Synergizes with WBP2 in enhancing PGR activity. (Microbial infection) Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. |
| Protein Name | Ubiquitin-Protein Ligase E3aE6ap Ubiquitin-Protein LigaseHect-Type Ubiquitin Transferase E3aHuman Papillomavirus E6-Associated ProteinOncogenic Protein-Associated Protein E6-ApRenal Carcinoma Antigen Ny-Ren-54 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative CLIA Names | Ubiquitin-Protein Ligase E3a CLIA kitE6ap Ubiquitin-Protein Ligase CLIA kitHect-Type Ubiquitin Transferase E3a CLIA kitHuman Papillomavirus E6-Associated Protein CLIA kitOncogenic Protein-Associated Protein E6-Ap CLIA kitRenal Carcinoma Antigen Ny-Ren-54 CLIA kitUBE3A CLIA kitE6AP CLIA kitEPVE6AP CLIA kitHPVE6A CLIA kit |
| Specificity | This kit recognizes Human UBE3A in samples. No significant cross-reactivity or interference between Human UBE3A and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-106 | 99 |
| EDTA plasma(n=8) | 83-99 | 90 |
| Cell culture media(n=8) | 96-113 | 103 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 186.53 | 343.31 | 1638.06 | 198.04 | 310.29 | 1602.45 |
| Standard deviation | 18.26 | 24.07 | 167.90 | 20.30 | 32.08 | 177.07 |
| CV (%) | 9.79 | 7.01 | 10.25 | 10.25 | 10.34 | 11.05 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
