| Short Description : | Recombinant-Human UBE2I/UBC9-N-GST protein was developed from e.coli and has a target region of N-GST. For use in research applications. |
| Applications: | ELISA/WB |
| Host: | E.coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Supplied as a 0.22 Mu m filtered solution in 50mM HEPES, 150mM NaCl, pH 7.5. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin: | < 1 EU/µg as determined by LAL test. |
| Gene Symbol: | UBE2I |
| Gene ID: | 7329 |
| Uniprot ID: | UBC9_HUMAN |
| Immunogen: | Homo sapiens (Human) |
| Immunogen Region: | Met1-Ser158 |
| Post Translational Modifications | Phosphorylation at Ser-71 significantly enhances SUMOylation activity. |
| Function | Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity. |
| Protein Name | Sumo-Conjugating Enzyme Ubc9Ring-Type E3 Sumo Transferase Ubc9Sumo-Protein LigaseUbiquitin Carrier Protein 9Ubiquitin Carrier Protein IUbiquitin-Conjugating Enzyme E2 IUbiquitin-Protein Ligase IP18 |
| Database Links | Reactome: R-HSA-1221632Reactome: R-HSA-196791Reactome: R-HSA-3065678Reactome: R-HSA-3108214Reactome: R-HSA-3232118Reactome: R-HSA-3232142Reactome: R-HSA-3899300Reactome: R-HSA-4085377Reactome: R-HSA-4090294Reactome: R-HSA-4551638Reactome: R-HSA-4570464Reactome: R-HSA-4615885Reactome: R-HSA-4655427Reactome: R-HSA-4755510Reactome: R-HSA-5693565Reactome: R-HSA-5693607Reactome: R-HSA-5696395Reactome: R-HSA-8866904Reactome: R-HSA-9615933Reactome: R-HSA-9683610Reactome: R-HSA-9694631Reactome: R-HSA-9735871Reactome: R-HSA-9793242Reactome: R-HSA-9833482Reactome: R-HSA-9843940Reactome: R-HSA-9856649 |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionMainly NuclearIn SpermatocytesLocalizes In Synaptonemal ComplexesRecruited By Bcl11a Into The Nuclear Body |
| Alternative Protein Names | Sumo-Conjugating Enzyme Ubc9 proteinRing-Type E3 Sumo Transferase Ubc9 proteinSumo-Protein Ligase proteinUbiquitin Carrier Protein 9 proteinUbiquitin Carrier Protein I proteinUbiquitin-Conjugating Enzyme E2 I proteinUbiquitin-Protein Ligase I proteinP18 proteinUBE2I proteinUBC9 proteinUBCE9 protein |
Information sourced from Uniprot.org