Human UBE2A (Ubiquitin Conjugating Enzyme E2A) Sandwich ELISA Kit (STJE0012893)
SPECIFICATIONS
General Information
| Short Description | This UBE2A Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.081ng/mL |
| Detection Limit | 0.312-20ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 4.5 hrs |
Target Information
| Gene Symbol | UBE2A |
| Gene ID | 7319 |
| Uniprot ID | UBE2A_HUMAN |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Additional Info
| Tissue Specificity | |
| Post Translational Modifications | Phosphorylation at Ser-120 by CDK9 increases activity towards histone H2B. |
| Function | E2 ubiquitin-conjugating enzyme that accepts ubiquitin from the ubiquitin-activating enzyme E1 and transfers it to a E3 ubiquitin-protein ligase. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Together with the E3 enzyme BRE1 (RNF20 and/or RNF40), plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. Involved in mitophagy by acting as a E2 ubiquitin-conjugating enzyme for PRKN. In association with the E3 enzyme UBR4, is involved in N-end rule-dependent protein degradation. In association with the E3 ubiquitin-protein ligase complex SIFI, inhibits the mitochondrial stress response by acting as a E2 ubiquitin-conjugating enzyme for UBR4 and KCMF1. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 AE2 Ubiquitin-Conjugating Enzyme ARad6 Homolog AHr6aHhr6aUbiquitin Carrier Protein AUbiquitin-Protein Ligase A |
| Database Links | Reactome: R-HSA-8866652Reactome: R-HSA-8866654Reactome: R-HSA-983168 |
| Cellular Localisation | Late EndosomeLysosome |
| Alternative ELISA Names | Ubiquitin-Conjugating Enzyme E2 A ELISA kitE2 Ubiquitin-Conjugating Enzyme A ELISA kitRad6 Homolog A ELISA kitHr6a ELISA kitHhr6a ELISA kitUbiquitin Carrier Protein A ELISA kitUbiquitin-Protein Ligase A ELISA kitUBE2A ELISA kitRAD6A ELISA kit |
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Information sourced from Uniprot.org