Human UBE2A protein (Recombinant) (N-GST-His) (STJP014516)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human UBE2A-N-GST-His protein was developed from e.coli and has a target region of N-GST-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Supplied as a 0.22 Mu m filtered solution in 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | < 1 EU/µg as determined by LAL test. |
Target Information
| Gene Symbol | UBE2A |
| Gene ID | 7319 |
| Uniprot ID | UBE2A_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Cys152 |
Additional Info
| Function | E2 ubiquitin-conjugating enzyme that accepts ubiquitin from the ubiquitin-activating enzyme E1 and transfers it to a E3 ubiquitin-protein ligase. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Together with the E3 enzyme BRE1 (RNF20 and/or RNF40), plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. Involved in mitophagy by acting as a E2 ubiquitin-conjugating enzyme for PRKN. In association with the E3 enzyme UBR4, is involved in N-end rule-dependent protein degradation. In association with the E3 ubiquitin-protein ligase complex SIFI, inhibits the mitochondrial stress response by acting as a E2 ubiquitin-conjugating enzyme for UBR4 and KCMF1. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 AE2 Ubiquitin-Conjugating Enzyme ARad6 Homolog AHr6aHhr6aUbiquitin Carrier Protein AUbiquitin-Protein Ligase A |
| Database Links | Reactome: R-HSA-8866652Reactome: R-HSA-8866654Reactome: R-HSA-983168 |
| Cellular Localisation | Late EndosomeLysosome |
| Alternative Protein Names | Ubiquitin-Conjugating Enzyme E2 A proteinE2 Ubiquitin-Conjugating Enzyme A proteinRad6 Homolog A proteinHr6a proteinHhr6a proteinUbiquitin Carrier Protein A proteinUbiquitin-Protein Ligase A proteinUBE2A proteinRAD6A protein |
Information sourced from Uniprot.org