Human Ubc2/UBE2A protein (Recombinant) (His-Tag) (STJP016695)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Ubc2/UBE2A-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% Glycerol, 1mM EDTA |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | UBE2A |
| Gene ID | 7319 |
| Uniprot ID | UBE2A_HUMAN |
| Accession Number | NP_003327 |
| Immunogen | Human |
| Immunogen Region | 1-152aa |
| Immunogen Sequence |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-120 by CDK9 increases activity towards histone H2B. |
| Function | E2 ubiquitin-conjugating enzyme that accepts ubiquitin from the ubiquitin-activating enzyme E1 and transfers it to a E3 ubiquitin-protein ligase. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Together with the E3 enzyme BRE1 (RNF20 and/or RNF40), plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. Involved in mitophagy by acting as a E2 ubiquitin-conjugating enzyme for PRKN. In association with the E3 enzyme UBR4, is involved in N-end rule-dependent protein degradation. In association with the E3 ubiquitin-protein ligase complex SIFI, inhibits the mitochondrial stress response by acting as a E2 ubiquitin-conjugating enzyme for UBR4 and KCMF1. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 AE2 Ubiquitin-Conjugating Enzyme ARad6 Homolog AHr6aHhr6aUbiquitin Carrier Protein AUbiquitin-Protein Ligase A |
| Database Links | Reactome: R-HSA-8866652Reactome: R-HSA-8866654Reactome: R-HSA-983168 |
| Cellular Localisation | Late EndosomeLysosome |
| Alternative Protein Names | Ubiquitin-Conjugating Enzyme E2 A proteinE2 Ubiquitin-Conjugating Enzyme A proteinRad6 Homolog A proteinHr6a proteinHhr6a proteinUbiquitin Carrier Protein A proteinUbiquitin-Protein Ligase A proteinUBE2A proteinRAD6A protein |
Information sourced from Uniprot.org