Human UBA5 protein (Recombinant) (His-Tag) (STJP017623)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human UBA5-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% Glycerol, 50mM NaCl |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | UBA5 |
| Gene ID | 79876 |
| Uniprot ID | UBA5_HUMAN |
| Accession Number | NP_079094 |
| Immunogen | Human |
| Immunogen Region | 1-404aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Widely expressed. |
| Function | E1-like enzyme which specifically catalyzes the first step in ufmylation. Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP. Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer. Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding. Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism. Ufmylation plays a key role in various processes, such as ribosome recycling, response to DNA damage, interferon response or reticulophagy (also called ER-phagy). Ufmylation is essential for erythroid differentiation of both megakaryocytes and erythrocytes. |
| Protein Name | Ubiquitin-Like Modifier-Activating Enzyme 5Ubiquitin-Activating Enzyme 5Thifp1Ufm1-Activating EnzymeUbiquitin-Activating Enzyme E1 Domain-Containing Protein 1 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusEndoplasmic Reticulum MembraneGolgi ApparatusLocalizes Mainly In The CytoplasmWhile It Localizes To The Nucleus In Presence Of Sumo2Interaction With Gabarapl2 Promotes Localization To The Endoplasmic Reticulum Membrane |
| Alternative Protein Names | Ubiquitin-Like Modifier-Activating Enzyme 5 proteinUbiquitin-Activating Enzyme 5 proteinThifp1 proteinUfm1-Activating Enzyme proteinUbiquitin-Activating Enzyme E1 Domain-Containing Protein 1 proteinUBA5 proteinUBE1DC1 protein |
Information sourced from Uniprot.org