Human TRPM7/LTRPC7 protein (Recombinant) (N-His) (STJP008510)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human TRPM7/LTRPC7-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | TRPM7 |
| Gene ID | 54822 |
| Uniprot ID | TRPM7_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Gly93-Gly431 |
Additional Info
| Function | Bifunctional protein that combines an ion channel with an intrinsic kinase domain, enabling it to modulate cellular functions either by conducting ions through the pore or by phosphorylating downstream proteins via its kinase domain. The channel is highly permeable to divalent cations, specifically calcium (Ca2+), magnesium (Mg2+) and zinc (Zn2+) and mediates their influx. Controls a wide range of biological processes such as Ca2(+), Mg(2+) and Zn(2+) homeostasis, vesicular Zn(2+) release channel and intracellular Ca(2+) signaling, embryonic development, immune responses, cell motility, proliferation and differentiation. The C-terminal alpha-kinase domain autophosphorylates cytoplasmic residues of TRPM7. In vivo, TRPM7 phosphorylates SMAD2, suggesting that TRPM7 kinase may play a role in activating SMAD signaling pathways. In vitro, TRPM7 kinase phosphorylates ANXA1 (annexin A1), myosin II isoforms and a variety of proteins with diverse cellular functions. TRPM7 channel, cleaved form: The cleaved channel exhibits substantially higher current and potentiates Fas receptor signaling. TRPM7 kinase, cleaved form: The C-terminal kinase domain can be cleaved from the channel segment in a cell-type-specific fashion. In immune cells, the TRPM7 kinase domain is clipped from the channel domain by caspases in response to Fas-receptor stimulation. The cleaved kinase fragments can translocate to the nucleus, and bind chromatin-remodeling complex proteins in a Zn(2+)-dependent manner to ultimately phosphorylate specific Ser/Thr residues of histones known to be functionally important for cell differentiation and embryonic development. |
| Protein Name | Transient Receptor Potential Cation Channel Subfamily M Member 7Channel-Kinase 1Long Transient Receptor Potential Channel 7Ltrpc-7Ltrpc7 Cleaved Into - Trpm7 Kinase - Cleaved FormM7ck - Trpm7 Channel - Cleaved Form |
| Database Links | Reactome: R-HSA-3295583 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCytoplasmic Vesicle MembraneLocalized Largely In Intracellular Zn(2+)-Storage VesiclesTrpm7 KinaseCleaved Form: Nucleus |
| Alternative Protein Names | Transient Receptor Potential Cation Channel Subfamily M Member 7 proteinChannel-Kinase 1 proteinLong Transient Receptor Potential Channel 7 proteinLtrpc-7 proteinLtrpc7 Cleaved Into - Trpm7 Kinase - Cleaved Form proteinM7ck - Trpm7 Channel - Cleaved Form proteinTRPM7 proteinCHAK1 proteinLTRPC7 protein |
Information sourced from Uniprot.org