| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
25.3pg/mL |
| Detection Limit: |
62.5-4000pg/mL |
| Short Description: |
This TRPM6 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates or other biological fluids.. |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
TRPM6 |
| Gene ID: |
140803 |
| Uniprot ID: |
TRPM6_HUMAN |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Highly expressed in kidney and colon. Isoform TRPM6a and isoform TRPM6b, are coexpressed with TRPM7 in kidney, and testis, and are also found in several cell lines of lung origin. Isoform TRPM6c is detected only in testis and in NCI-H510A small cell lung carcinoma cells. |
| Post Translational Modifications | Autophosphorylated.autophosphorylation controlls the protein kinase activity of TRPM6 towards their substrates. Autophosphorylation of Thr-1851 in the kinase domain is essential for the inhibitory effect of RACK1. The C-terminus of TRPM6 is proteolytically cleaved in vivo, in a cell type-specific fashion, releasing the kinase module from the transmembrane domain. The cleaved kinase fragments are translocated to the nucleus to phosphorylate histones and regulate gene expression. |
| Function | Bifunctional protein that combines an ion channel with an intrinsic kinase domain, enabling it to modulate cellular functions either by conducting ions through the pore or by phosphorylating downstream proteins via its kinase domain. Crucial for Mg(2+) homeostasis. Has an important role in epithelial Mg(2+) transport and in the active Mg(2+) absorption in the gut and kidney. However, whether TRPM6 forms functional homomeric channels by itself or functions primarily as a subunit of heteromeric TRPM6-TRPM7 channels, is still under debate. TRPM6 kinase, cleaved form: The C-terminal kinase domain can be cleaved from the channel segment in a cell-type-specific fashion. The cleaved kinase fragments can translocate to the nucleus, and bind chromatin-remodeling complex proteins to ultimately phosphorylate specific Ser/Thr residues of histones known to be functionally important for cell differentiation and development. |
| Protein Name | Transient Receptor Potential Cation Channel Subfamily M Member 6Channel Kinase 2Melastatin-Related Trp Cation Channel 6 Cleaved Into - Trpm6 Kinase - Cleaved Form |
| Database Links | Reactome: R-HSA-3295583 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinApical Cell MembraneTrpm6 Requires The Presence Of Trpm7 To Be Targeted To The Cell Membrane (In Hek 293 Cells)Trpm6 KinaseCleaved Form: Nucleus |
| Alternative ELISA Names | Transient Receptor Potential Cation Channel Subfamily M Member 6 ELISA kitChannel Kinase 2 ELISA kitMelastatin-Related Trp Cation Channel 6 Cleaved Into - Trpm6 Kinase - Cleaved Form ELISA kitTRPM6 ELISA kitCHAK2 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
