• Biotinylated Human TPBG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human TPBG is greater than 95% as determined by SEC-HPLC.
  • Immobilized Anti-TPBG Antibody, hFc Tag at 2 Mu g/ml (100 Mu l/well) on the plate. Dose response curve for Biotinylated Human TPBG, His Tag with the EC50 of 84. 3ng/ml determined by ELISA.

Human TPBG/5T4 protein (Recombinant) (C-His-Avi) {Biotin} (STJP001536)

SKU:
STJP001536

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human TPBG/5T4-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: TPBG
Gene ID: 7162
Uniprot ID: TPBG_HUMAN
Immunogen Region: Ser32-Ser355
Immunogen: DNA sequence encoding Human TPBG/5T4 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications Highly glycosylated.
Function May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
Protein Name Trophoblast Glycoprotein
5t4 Oncofetal Antigen
5t4 Oncofetal Trophoblast Glycoprotein
5t4 Oncotrophoblast Glycoprotein
M6p1
Wnt-Activated Inhibitory Factor 1
Waif1
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Protein Names Trophoblast Glycoprotein
5t4 Oncofetal Antigen protein
5t4 Oncofetal Trophoblast Glycoprotein
5t4 Oncotrophoblast Glycoprotein
M6p1 protein
Wnt-Activated Inhibitory Factor 1 protein
Waif1 protein
TPBG protein
5T4 protein

Information sourced from Uniprot.org

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