Human TP53BP1 protein (Recombinant) (N-His) (STJP005267)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human TP53BP1-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | TP53BP1 |
| Gene ID | 7158 |
| Uniprot ID | TP53B_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Ala1614-His1972 |
Additional Info
| Function | Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis. Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1. In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs. Participates in the repair and the orientation of the broken DNA ends during CSR. In contrast, it is not required for classic NHEJ and V(D)J recombination. Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1. |
| Protein Name | Tp53-Binding Protein 153bp1P53-Binding Protein 1P53bp1 |
| Database Links | Reactome: R-HSA-3232118Reactome: R-HSA-5693565Reactome: R-HSA-5693571Reactome: R-HSA-5693607Reactome: R-HSA-69473 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreLocalizes To The Nucleus In Absence Of Dna DamageFollowing Dna DamageRecruited To Sites Of Dna DamageSuch As Double Stand Breaks (Dsbs): Recognizes And Binds Histone H2a Monoubiquitinated At 'Lys-15' (H2ak15ub) And Histone H4 Dimethylated At 'Lys-20' (H4k20me2)Two Histone Marks That Are Present At Dsbs SitesAssociated With Kinetochores During Mitosis |
| Alternative Protein Names | Tp53-Binding Protein 1 protein53bp1 proteinP53-Binding Protein 1 proteinP53bp1 proteinTP53BP1 protein |
Information sourced from Uniprot.org