| Post Translational Modifications | Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment. Phosphorylation at Ser-506 by CK2 increases binding to supercoiled DNA and sensitivity to camptothecin. |
| Function | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the BMAL1 promoter. |
| Protein Name | Dna Topoisomerase 1Dna Topoisomerase I |
| Database Links | Reactome: R-HSA-4615885 |
| Cellular Localisation | NucleusNucleolusNucleoplasmDiffuse Nuclear Localization With Some Enrichment In NucleoliOn Cpt TreatmentCleared From Nucleoli Into NucleoplasmSumoylated Forms Found In Both Nucleoplasm And Nucleoli |
| Alternative Protein Names | Dna Topoisomerase 1 proteinDna Topoisomerase I proteinTOP1 protein |
Information sourced from Uniprot.org
