Human TNFRSF9 (Tumor Necrosis Factor Receptor Superfamily, Member 9) Sandwich ELISA Kit (STJE0019887)

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STJE0019887
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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.06 ng/mL
Detection Limit: 0.16-10 ng/mL
Short Description : This TNFRSF9 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: TNFRSF9
Gene ID: 3604
Uniprot ID: TNR9_HUMAN
Specificity: This kit recognizes Human TNFRSF9 in samples. No significant cross-reactivity or interference between Human TNFRSF9 and analogues was observed.
Sample Type: serum, plasma and other biological fluids
Tissue Specificity Expressed on the surface of activated T-cells.
Function Receptor for TNFSF9/4-1BBL. Conveys a signal that enhances CD8(+) T-cell survival, cytotoxicity, and mitochondrial activity, thereby promoting immunity against viruses and tumors (Probable).
Protein Name Tumor Necrosis Factor Receptor Superfamily Member 9
4-1bb Ligand Receptor
Cdw137
T-Cell Antigen 4-1bb Homolog
T-Cell Antigen Ila
Cd Antigen Cd137
Database Links Reactome: R-HSA-5669034
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative ELISA Names Tumor Necrosis Factor Receptor Superfamily Member 9 ELISA kit
4-1bb Ligand Receptor ELISA kit
Cdw137 ELISA kit
T-Cell Antigen 4-1bb Homolog ELISA kit
T-Cell Antigen Ila ELISA kit
Cd Antigen Cd137 ELISA kit
TNFRSF9 ELISA kit
CD137 ELISA kit
ILA ELISA kit
Specificity This kit recognizes Human TNFRSF9 in samples. No significant cross-reactivity or interference between Human TNFRSF9 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-102 97
EDTA plasma (n=8) 87-101 93
Cell culture media (n=8) 93-108 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.55 1.26 4.66 0.60 1.32 4.36
Standard deviation 0.03 0.06 0.15 0.04 0.07 0.15
CV (%) 6.10 4.75 3.16 6.11 5.23 3.50

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