| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.06 ng/mL |
| Detection Limit: | 0.16-10 ng/mL |
| Short Description : | This TNFRSF9 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | TNFRSF9 |
| Gene ID: | 3604 |
| Uniprot ID: | TNR9_HUMAN |
| Specificity: | This kit recognizes Human TNFRSF9 in samples. No significant cross-reactivity or interference between Human TNFRSF9 and analogues was observed. |
| Sample Type: | serum, plasma and other biological fluids |
| Tissue Specificity | Expressed on the surface of activated T-cells. |
| Function | Receptor for TNFSF9/4-1BBL. Conveys a signal that enhances CD8(+) T-cell survival, cytotoxicity, and mitochondrial activity, thereby promoting immunity against viruses and tumors (Probable). |
| Protein Name | Tumor Necrosis Factor Receptor Superfamily Member 9 4-1bb Ligand Receptor Cdw137 T-Cell Antigen 4-1bb Homolog T-Cell Antigen Ila Cd Antigen Cd137 |
| Database Links | Reactome: R-HSA-5669034 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein |
| Alternative ELISA Names | Tumor Necrosis Factor Receptor Superfamily Member 9 ELISA kit 4-1bb Ligand Receptor ELISA kit Cdw137 ELISA kit T-Cell Antigen 4-1bb Homolog ELISA kit T-Cell Antigen Ila ELISA kit Cd Antigen Cd137 ELISA kit TNFRSF9 ELISA kit CD137 ELISA kit ILA ELISA kit |
| Specificity | This kit recognizes Human TNFRSF9 in samples. No significant cross-reactivity or interference between Human TNFRSF9 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 92-102 | 97 |
| EDTA plasma (n=8) | 87-101 | 93 |
| Cell culture media (n=8) | 93-108 | 100 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.55 | 1.26 | 4.66 | 0.60 | 1.32 | 4.36 |
| Standard deviation | 0.03 | 0.06 | 0.15 | 0.04 | 0.07 | 0.15 |
| CV (%) | 6.10 | 4.75 | 3.16 | 6.11 | 5.23 | 3.50 |