| Post Translational Modifications | Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF). This response is dependent on an intact actin microfilament system. Dephosphorylated by PTPRJ. |
| Function | TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling. With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus. May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions. |
| Protein Name | Tight Junction Protein Zo-1Tight Junction Protein 1Zona Occludens Protein 1Zonula Occludens Protein 1 |
| Database Links | Reactome: R-HSA-191650Reactome: R-HSA-2028269Reactome: R-HSA-351906Reactome: R-HSA-8935964Reactome: R-HSA-9705677 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmic SideCell JunctionTight JunctionGap JunctionCell ProjectionPodosomeMoves From The Cytoplasm To The Cell Membrane Concurrently With Cell-Cell ContactAt Podosomal SitesIs Predominantly Localized In The Ring Structure Surrounding The Actin CoreColocalizes With Spef1 At Sites Of Cell-Cell Contact In Intestinal Epithelial Cells |
| Alternative ELISA Names | Tight Junction Protein Zo-1 ELISA kitTight Junction Protein 1 ELISA kitZona Occludens Protein 1 ELISA kitZonula Occludens Protein 1 ELISA kitTJP1 ELISA kitZO1 ELISA kit |
| Specificity | This kit recognizes Human TJP1 in samples. No significant cross-reactivity or interference between Human TJP1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-100 | 93 |
| EDTA plasma (n=8) | 96-108 | 101 |
| Cell culture media (n=8) | 90-107 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.50 | 1.29 | 4.40 | 0.55 | 1.17 | 4.57 |
| Standard deviation | 0.03 | 0.06 | 0.24 | 0.03 | 0.05 | 0.14 |
| CV (%) | 6.00 | 4.65 | 5.45 | 5.45 | 4.27 | 3.06 |
