Human THPO (Thrombopoietin) CLIA Kit (STJC0000718)

Human THPO (Thrombopoietin) CLIA Kit (STJC0000718)

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STJC0000718
£180.50 - £462.50
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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 7.5pg/mL
Detection Limit: 12.5~800pg/mL
Short Description : This human THPO kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: THPO
Gene ID: 7066
Uniprot ID: TPO_HUMAN
Specificity: This kit recognizes Human TPO in samples. No significant cross-reactivity or interference between Human TPO and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Protein Name Thrombopoietin
C-Mpl Ligand
Ml
Megakaryocyte Colony-Stimulating Factor
Megakaryocyte Growth And Development Factor
Mgdf
Myeloproliferative Leukemia Virus Oncogene Ligand
Database Links Reactome: R-HSA-76009
Cellular Localisation Secreted
Alternative CLIA Names Thrombopoietin CLIA kit
C-Mpl Ligand CLIA kit
Ml CLIA kit
Megakaryocyte Colony-Stimulating Factor CLIA kit
Megakaryocyte Growth And Development Factor CLIA kit
Mgdf CLIA kit
Myeloproliferative Leukemia Virus Oncogene Ligand CLIA kit
THPO CLIA kit
MGDF CLIA kit
Specificity This kit recognizes Human TPO in samples. No significant cross-reactivity or interference between Human TPO and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-101 94
EDTA plasma(n=8) 92-104 99
Cell culture media(n=8) 95-106 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 39.87 87.91 370.41 38.21 95.78 370.24
Standard deviation 3.97 7.11 30.67 4.28 8.97 29.55
CV (%) 9.96 8.09 8.28 11.20 9.37 7.98

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