| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.109ng/mL |
| Detection Limit: | 0.312-20ng/mL |
| Short Description : | This TFAM Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids.. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | TFAM |
| Gene ID: | 7019 |
| Uniprot ID: | TFAM_HUMAN |
| Sample Type: | tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Phosphorylation by PKA within the HMG box 1 impairs DNA binding and promotes degradation by the AAA+ Lon protease. |
| Function | Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. |
| Protein Name | Transcription Factor A - Mitochondrial Mttfa Mitochondrial Transcription Factor 1 Mttf1 Transcription Factor 6 Tcf-6 Transcription Factor 6-Like 2 |
| Database Links | Reactome: R-HSA-163282 Reactome: R-HSA-2151201 Reactome: R-HSA-9837999 |
| Cellular Localisation | Mitochondrion Mitochondrion Matrix Mitochondrion Nucleoid |
| Alternative ELISA Names | Transcription Factor A - Mitochondrial ELISA kit Mttfa ELISA kit Mitochondrial Transcription Factor 1 ELISA kit Mttf1 ELISA kit Transcription Factor 6 ELISA kit Tcf-6 ELISA kit Transcription Factor 6-Like 2 ELISA kit TFAM ELISA kit TCF6 ELISA kit TCF6L2 ELISA kit |
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Information sourced from Uniprot.org