Function | Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The TF:VIIa complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. |
Protein Name | Tissue FactorTfCoagulation Factor IiiThromboplastinCd Antigen Cd142 |
Database Links | Reactome: R-HSA-140834Reactome: R-HSA-9031628 |
Cellular Localisation | Isoform 1: MembraneSingle-Pass Type I Membrane ProteinIsoform 2: Secreted |
Alternative ELISA Names | Tissue Factor ELISA kitTf ELISA kitCoagulation Factor Iii ELISA kitThromboplastin ELISA kitCd Antigen Cd142 ELISA kitF3 ELISA kit |
Specificity | This kit recognizes Human F3/TF in samples. No significant cross-reactivity or interference between Human F3/TF and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 86-99 | 92 |
EDTA plasma (n=8) | 92-106 | 99 |
Cell culture media (n=8) | 92-104 | 97 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 27.10 | 60.96 | 237.83 | 27.43 | 58.54 | 231.58 |
Standard deviation | 1.80 | 2.58 | 8.92 | 1.62 | 2.41 | 11.90 |
CV (%) | 6.64 | 4.23 | 3.75 | 5.91 | 4.12 | 5.14 |