Human TACE/ADAM17 (Disintegrin And Metalloproteinase Domain-Containing Protein 17) Antibody Pair (STJAP00444)
General Information
| Short Description | This TACE/ADAM17 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | ADAM17 |
| Gene ID | 6868 |
| Uniprot ID | ADA17_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human TACE/ADAM17 protien.Detection Immunogen: Recombinant Human TACE/ADAM17 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | The precursor is cleaved by a furin endopeptidase. Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. Phosphorylation at Thr-735 by MAPK14 is required for ADAM17-mediated ectodomain shedding. |
| Function | Transmembrane metalloprotease which mediates the ectodomain shedding of a myriad of transmembrane proteins including adhesion proteins, growth factor precursors and cytokines important for inflammation and immunity. Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2. Plays a role in hemostasis through shedding of GP1BA, the platelet glycoprotein Ib alpha chain. Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3. Mediates the proteolytic cleavage of IL6R, leading to the release of secreted form of IL6R. Mediates the proteolytic cleavage and shedding of FCGR3A upon NK cell stimulation, a mechanism that allows for increased NK cell motility and detachment from opsonized target cells. Cleaves TREM2, resulting in shedding of the TREM2 ectodomain. |
| Protein Name | Disintegrin And Metalloproteinase Domain-Containing Protein 17Adam 17Snake Venom-Like ProteaseTnf-Alpha ConvertaseTnf-Alpha-Converting EnzymeCd Antigen Cd156b |
| Database Links | Reactome: R-HSA-1251985Reactome: R-HSA-1442490Reactome: R-HSA-177929Reactome: R-HSA-193692Reactome: R-HSA-2122948Reactome: R-HSA-2644606Reactome: R-HSA-2660826Reactome: R-HSA-2691232Reactome: R-HSA-2894862Reactome: R-HSA-5362798Reactome: R-HSA-75893Reactome: R-HSA-9662834Reactome: R-HSA-982772 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Pair Names | Disintegrin And Metalloproteinase Domain-Containing Protein 17 antibody pairAdam 17 antibody pairSnake Venom-Like Protease antibody pairTnf-Alpha Convertase antibody pairTnf-Alpha-Converting Enzyme antibody pairCd Antigen Cd156b antibody pairADAM17 antibody pairCSVP antibody pairTACE antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human TACE/ADAM17 (Disintegrin And Metalloproteinase Domain-Containing Protein 17) Antibody Pair (STJAP00444)
This antibody pair detects human TACE/ADAM17 (Disintegrin And Metalloproteinase Domain-Containing Protein 17), corresponding to UniProt entry P78536. Reactivity has been confirmed for human samples only.
The capture antibody is supplied unconjugated and the detection antibody is supplied biotinylated, both at 0.5 mg/mL. Both antibodies are mouse-derived and were purified by Protein A or G affinity chromatography.
This pair has been validated for use in ELISA. It is intended for the detection of human TACE/ADAM17 in serum, plasma, and other biological fluids.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
Both the capture and detection antibodies were raised against recombinant human TACE/ADAM17 protein. Both antibodies are mouse-derived.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.