• Human SYNPO (Synaptopodin) CLIA Kit (STJC0000860)

Human SYNPO (Synaptopodin) CLIA Kit (STJC0000860)

SKU:
STJC0000860

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human SYNPO kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: SYNPO
Gene ID: 11346
Uniprot ID: SYNPO_HUMAN
Specificity: This kit recognizes Human SYNPO in samples. No significant cross-reactivity or interference between Human SYNPO and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in cerebral cortex.
Post Translational Modifications O-glycosylated.
Function Actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity.
Protein Name Synaptopodin
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Junction
Tight Junction
Perikaryon
Cell Projection
Dendritic Spine
Postsynaptic Density
Synapse
Cytosol
Localized At The Tight Junction Of Cells
In Brain
Localized To The Postsynaptic Densities And In The Perikarya
Associated With Dendritic Spines Of A Subset Of Synapses
Alternative CLIA Names Synaptopodin CLIA kit
SYNPO CLIA kit
KIAA1029 CLIA kit
Specificity This kit recognizes Human SYNPO in samples. No significant cross-reactivity or interference between Human SYNPO and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 97-111 105
EDTA plasma(n=8) 96-112 102
Cell culture media(n=8) 89-103 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 108.03 170.04 869.89 106.51 169.09 869.70
Standard deviation 11.99 19.52 65.94 13.62 19.09 66.10
CV (%) 11.10 11.48 7.58 12.79 11.29 7.60
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance