Function | Destroys radicals which are normally produced within the cells and which are toxic to biological systems. |
Protein Name | Superoxide Dismutase Cu-ZnSuperoxide Dismutase 1Hsod1 |
Database Links | Reactome: R-HSA-114608Reactome: R-HSA-3299685Reactome: R-HSA-8950505 |
Cellular Localisation | CytoplasmNucleusPredominantly CytoplasmicThe Pathogenic Variants Als1 Arg-86 And Ala-94 Gradually Aggregates And Accumulates In Mitochondria |
Alternative ELISA Names | Superoxide Dismutase Cu-Zn ELISA kitSuperoxide Dismutase 1 ELISA kitHsod1 ELISA kitSOD1 ELISA kit |
Specificity | This kit recognizes SOD1 in samples. No significant cross-reactivity or interference between SOD1 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8℃ |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 96-108 | 102 |
EDTA plasma(n=8) | 88-101 | 94 |
Cell culture media(n=8) | 91-105 | 97 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 209.30 | 367.40 | 1835.40 | 196.20 | 331.30 | 1975.00 |
Standard deviation | 13.20 | 14.70 | 55.10 | 12.80 | 16.60 | 75.10 |
CV (%) | 6.31 | 4.00 | 3.00 | 6.52 | 5.01 | 3.80 |