Human SMAD5 protein (Recombinant) (N-His) (STJP005905)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human SMAD5-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | SMAD5 |
| Gene ID | 4090 |
| Uniprot ID | SMAD5_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Gly210-Val464 |
Additional Info
| Post Translational Modifications | Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. |
| Function | Transcriptional regulator that plays a role in various cellular processes including embryonic development, cell differentiation, angiogenesis and tissue homeostasis. Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form a heteromeric complex which translocates into the nucleus acting as transcription factor. In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network. Non-phosphorylated SMAD5 has a cytoplasmic role in energy metabolism regulation by promoting mitochondrial respiration and glycolysis in response to cytoplasmic pH changes. Mechanistically, interacts with hexokinase 1/HK1 and thereby accelerates glycolysis. |
| Protein Name | Mothers Against Decapentaplegic Homolog 5Mad Homolog 5Mothers Against Dpp Homolog 5Jv5-1Smad Family Member 5Smad 5Smad5Hsmad5 |
| Database Links | Reactome: R-HSA-201451 |
| Cellular Localisation | CytoplasmNucleusMitochondrionCytoplasmic In The Absence Of LigandMigrates To The Nucleus When Complexed With Smad4 |
| Alternative Protein Names | Mothers Against Decapentaplegic Homolog 5 proteinMad Homolog 5 proteinMothers Against Dpp Homolog 5 proteinJv5-1 proteinSmad Family Member 5 proteinSmad 5 proteinSmad5 proteinHsmad5 proteinSMAD5 proteinMADH5 protein |
Information sourced from Uniprot.org