Human SIAH2 protein (Recombinant) (N-His) (STJP009578)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human SIAH2-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | SIAH2 |
| Gene ID | 6478 |
| Uniprot ID | SIAH2_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Ser68-Pro324 |
Additional Info
| Post Translational Modifications | Phosphorylated at Ser-28 by MAPK14, which mediates the degradation by the proteasome of EGLN3. Phosphorylated at Ser-28 by DYRK2.this increases the ubiquitin ligase activity and promotes degradation of EGLN3. |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (GPS2, POU2AF1, PML, NCOR1), a cell surface receptor (DCC), an antiapoptotic protein (BAG1), and a protein involved in synaptic vesicle function in neurons (SYP). Mediates ubiquitination and proteasomal degradation of DYRK2 in response to hypoxia. It is thereby involved in apoptosis, tumor suppression, cell cycle, transcription and signaling processes. Has some overlapping function with SIAH1. Triggers the ubiquitin-mediated degradation of TRAF2, whereas SIAH1 does not. Promotes monoubiquitination of SNCA. Regulates cellular clock function via ubiquitination of the circadian transcriptional repressors NR1D1 and NR1D2 leading to their proteasomal degradation. Plays an important role in mediating the rhythmic degradation/clearance of NR1D1 and NR1D2 contributing to their circadian profile of protein abundance. Mediates ubiquitination and degradation of EGLN2 and EGLN3 in response to the unfolded protein response (UPR), leading to their degradation and subsequent stabilization of ATF4. Also part of the Wnt signaling pathway in which it mediates the Wnt-induced ubiquitin-mediated proteasomal degradation of AXIN1. |
| Protein Name | E3 Ubiquitin-Protein Ligase Siah2Ring-Type E3 Ubiquitin Transferase Siah2Seven In Absentia Homolog 2Siah-2Hsiah2 |
| Database Links | Reactome: R-HSA-373752Reactome: R-HSA-5689880Reactome: R-HSA-977225Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusPredominantly CytoplasmicPartially Nuclear |
| Alternative Protein Names | E3 Ubiquitin-Protein Ligase Siah2 proteinRing-Type E3 Ubiquitin Transferase Siah2 proteinSeven In Absentia Homolog 2 proteinSiah-2 proteinHsiah2 proteinSIAH2 protein |
Information sourced from Uniprot.org