Human SHBG (Sex Hormone Binding Globulin) Sandwich ELISA Kit (STJE0019905)

Name: Human SHBG (Sex Hormone Binding Globulin) Sandwich ELISA Kit (STJE0019905)
SKU: STJE0019905
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0019905

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Short Description	This SHBG Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	4.28 fmol/mL
Detection Limit	7.81-500 fmol/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	SHBG
Gene ID	6462
Uniprot ID	SHBG_HUMAN
Specificity	This kit recognizes Human SHBG in samples. No significant cross-reactivity or interference between Human SHBG and analogues was observed.
Sample Type	serum, plasma and other biological fluids

Tissue Specificity	Isoform 1 and isoform 2 are present in liver and testis.
Post Translational Modifications	Variant Asn-356 contains one N-linked (GlcNAc..) at position 356.
Function	Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
Protein Name	Sex Hormone-Binding Globulin Shbg Sex Steroid-Binding Protein Sbp Testis-Specific Androgen-Binding Protein Abp Testosterone-Estradiol-Binding Globulin Tebg Testosterone-Estrogen-Binding Globulin
Database Links
Cellular Localisation	Secreted In Testis It Is Synthesized By The Sertoli Cells Secreted Into The Lumen Of The Seminiferous Tubule And Transported To The Epididymis
Alternative ELISA Names	Sex Hormone-Binding Globulin ELISA kit Shbg ELISA kit Sex Steroid-Binding Protein ELISA kit Sbp ELISA kit Testis-Specific Androgen-Binding Protein ELISA kit Abp ELISA kit Testosterone-Estradiol-Binding Globulin ELISA kit Tebg ELISA kit Testosterone-Estrogen-Binding Globulin ELISA kit SHBG ELISA kit
Specificity	This kit recognizes Human SHBG in samples. No significant cross-reactivity or interference between Human SHBG and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (fmol/mL)	27.07	53.05	181.43	26.49	52.67	187.50
Standard deviation	1.52	2.98	6.95	1.84	3.00	7.44
CV (%)	5.61	5.62	3.83	6.96	5.70	3.97