Human sgp130 (Interleukin-6 Receptor Subunit Beta) Antibody Pair (STJAP00375)
General Information
| Short Description | This sgp130 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | IL6ST |
| Gene ID | 3572 |
| Uniprot ID | IL6RB_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human sgp130 protien.Detection Immunogen: Recombinant Human sgp130 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Phosphorylation of Ser-782 down-regulates cell surface expression. Heavily N-glycosylated. Glycosylation is required for protein stability and localization in plasma membrane but not for ligand binding. |
| Function | Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates the intracellular JAK-MAPK and JAK-STAT3 signaling pathways. That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3. In parallel, the IL6 signaling pathway induces the expression of two cytokine receptor signaling inhibitors, SOCS1 and SOCS3, which inhibit JAK and terminate the activity of the IL6 signaling pathway as a negative feedback loop. Also activates the yes-associated protein 1 (YAP) and NOTCH pathways to control inflammation-induced epithelial regeneration, independently of STAT3. Acts as a receptor for the neuroprotective peptide humanin as part of a complex with IL27RA/WSX1 and CNTFR. Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism. Has a role in embryonic development. Essential for survival of motor and sensory neurons and for differentiation of astrocytes. Required for expression of TRPA1 in nociceptive neurons. Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation. Required for normal trabecular bone mass and cortical bone composition. Isoform 2: Binds to the soluble IL6:sIL6R complex (hyper-IL6), thereby blocking IL6 trans-signaling. Inhibits sIL6R-dependent acute phase response. Also blocks IL11 cluster signaling through IL11R. |
| Protein Name | Interleukin-6 Receptor Subunit BetaIl-6 Receptor Subunit BetaIl-6r Subunit BetaIl-6r-BetaIl-6rbCdw130Interleukin-6 Signal TransducerMembrane Glycoprotein 130Gp130Oncostatin-M Receptor Subunit AlphaCd Antigen Cd130 |
| Database Links | Reactome: R-HSA-1059683Reactome: R-HSA-110056 P40189-1Reactome: R-HSA-112411 P40189-1Reactome: R-HSA-6788467Reactome: R-HSA-8984722Reactome: R-HSA-9020956 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 2: Secreted |
| Alternative Antibody Pair Names | Interleukin-6 Receptor Subunit Beta antibody pairIl-6 Receptor Subunit Beta antibody pairIl-6r Subunit Beta antibody pairIl-6r-Beta antibody pairIl-6rb antibody pairCdw130 antibody pairInterleukin-6 Signal Transducer antibody pairMembrane Glycoprotein 130 antibody pairGp130 antibody pairOncostatin-M Receptor Subunit Alpha antibody pairCd Antigen Cd130 antibody pairIL6ST antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human sgp130 (Interleukin-6 Receptor Subunit Beta) Antibody Pair (STJAP00375)
This antibody pair detects human sgp130, also known as Interleukin-6 Receptor Subunit Beta (UniProt P40189), and is validated for human samples only. No cross-reactivity data for other species is provided.
The capture antibody is unconjugated and mouse-hosted, purified by Protein A or G, while the detection antibody is biotin-conjugated and goat-hosted, purified by antigen affinity. Both components are supplied at 0.5 mg/mL.
This pair is validated for use in ELISA. No other assay formats are listed in the product specifications.
This antibody pair has been indicated for use with serum, plasma, and other biological fluids. This product is intended for research use only and is not validated for diagnostic purposes.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, while the detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5. Both components should be stored at -20°C for long-term storage, and repeated freeze-thaw cycles should be avoided.
Both the capture and detection antibodies were raised against recombinant human sgp130 protein. No specific immunogen region is defined in the product specifications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.